Rubella virus serology:detection of residual lipoprotein inhibitors of haemagglutination using sensitive indicator arboviruses.
Identifieur interne : 002A89 ( Main/Exploration ); précédent : 002A88; suivant : 002A90Rubella virus serology:detection of residual lipoprotein inhibitors of haemagglutination using sensitive indicator arboviruses.
Auteurs : K F ShortridgeSource :
- Journal of Clinical Pathology [ 0021-9746 ] ; 1977-05.
English descriptors
- Teeft :
- Ageing, Arbovirus, Arbovirus antibody, Assay, Fresh sera, Fresh serum, Haemagglutination, Haemagglutination inhibition, Hong kong, Immune rubella sera, Inhibitor, Inhibitory activity, Japanese encephalitis, Kaolin, Lipoprotein, Lipoprotein removal, Residual, Residual lipoprotein inhibitors, Rubella, Rubella sera, Rubella virus, Rubella virus infection, Rubella virus serology, Sera serum, Serum, Shortridge, Titrated, Titre, Virus, West nile.
Abstract
Immune and non-immune rubella sera were examined in the haemagglutination inhibition (HAI) test to evaluate the removal of lipoprotein non-specific inhibitors (NSI) of haemagglutination after kaolin and manganous chloride/heparin treatments. The sera were titrated fresh or after lipoprotein deterioration brought about by ageing the samples at 4 degrees C for various periods of time and by freezing at -20 degrees C with subsequent thawing. Deterioration was seen as altered electrophoretic mobility while the lipoproteins in treated sera were detected by indicator arboviruses whose haemagglutination is known to be strongly inhibited by the native macromolecules. After both treatments, notably manganous chloride/heparin, residual NSI activity was detected in deteriorated samples, particularly with group B arboviruses such as Japanese encephalitis and west Nile viruses but generally less so or not at all with the group A arboviruses employed. Absolutely fresh sera are considered highly desirable for rubella virus HAI assay, and it is suggested that the efficiency of lipoprotein NSI removal regardless of treatment protocol could be monitored in parallel HAI tests using carefully chosen indicator arboviruses. This could be done in conjunction with density gradient centrifugation of doubtful sera should ultracentrifuging facilities be available. The suitability of the monitoring procedure would be dependent to some extent on whether certain arboviruses are known to be endemic in a particular area.
Url:
- https://api.istex.fr/ark:/67375/NVC-BP37HTG3-D/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC476431
DOI: 10.1136/jcp.30.5.409
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001517
- to stream Istex, to step Curation: 001517
- to stream Istex, to step Checkpoint: 001667
- to stream Main, to step Merge: 002C50
- to stream Pmc, to step Corpus: 000336
- to stream Pmc, to step Curation: 000336
- to stream Pmc, to step Checkpoint: 000E09
- to stream Ncbi, to step Merge: 001327
- to stream Ncbi, to step Curation: 001327
- to stream Ncbi, to step Checkpoint: 001327
- to stream Main, to step Merge: 002B93
- to stream Main, to step Curation: 002A89
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rubella virus serology:detection of residual lipoprotein inhibitors of haemagglutination using sensitive indicator arboviruses.</title><author wicri:is="90%"><name sortKey="Shortridge, K F" sort="Shortridge, K F" uniqKey="Shortridge K" first="K F" last="Shortridge">K F Shortridge</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:DD8C76345B1ECF4527F15355BE9C5E11C51A5454</idno><date when="1977" year="1977">1977</date><idno type="doi">10.1136/jcp.30.5.409</idno><idno type="url">https://api.istex.fr/ark:/67375/NVC-BP37HTG3-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001517</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001517</idno><idno type="wicri:Area/Istex/Curation">001517</idno><idno type="wicri:Area/Istex/Checkpoint">001667</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001667</idno><idno type="wicri:doubleKey">0021-9746:1977:Shortridge K:rubella:virus:serology</idno><idno type="wicri:Area/Main/Merge">002C50</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC476431</idno><idno type="RBID">PMC:476431</idno><idno type="wicri:Area/Pmc/Corpus">000336</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000336</idno><idno type="wicri:Area/Pmc/Curation">000336</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000336</idno><idno type="wicri:Area/Pmc/Checkpoint">000E09</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000E09</idno><idno type="wicri:Area/Ncbi/Merge">001327</idno><idno type="wicri:Area/Ncbi/Curation">001327</idno><idno type="wicri:Area/Ncbi/Checkpoint">001327</idno><idno type="wicri:doubleKey">0021-9746:1977:Shortridge K:rubella:virus:serology</idno><idno type="wicri:Area/Main/Merge">002B93</idno><idno type="wicri:Area/Main/Curation">002A89</idno><idno type="wicri:Area/Main/Exploration">002A89</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Rubella virus serology:detection of residual lipoprotein inhibitors of haemagglutination using sensitive indicator arboviruses.</title><author wicri:is="90%"><name sortKey="Shortridge, K F" sort="Shortridge, K F" uniqKey="Shortridge K" first="K F" last="Shortridge">K F Shortridge</name></author></analytic><monogr></monogr><series><title level="j">Journal of Clinical Pathology</title><title level="j" type="abbrev">J Clin Pathol</title><idno type="ISSN">0021-9746</idno><idno type="eISSN">1472-4146</idno><imprint><publisher>BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><date type="published" when="1977-05">1977-05</date><biblScope unit="volume">30</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="409">409</biblScope></imprint><idno type="ISSN">0021-9746</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9746</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Ageing</term><term>Arbovirus</term><term>Arbovirus antibody</term><term>Assay</term><term>Fresh sera</term><term>Fresh serum</term><term>Haemagglutination</term><term>Haemagglutination inhibition</term><term>Hong kong</term><term>Immune rubella sera</term><term>Inhibitor</term><term>Inhibitory activity</term><term>Japanese encephalitis</term><term>Kaolin</term><term>Lipoprotein</term><term>Lipoprotein removal</term><term>Residual</term><term>Residual lipoprotein inhibitors</term><term>Rubella</term><term>Rubella sera</term><term>Rubella virus</term><term>Rubella virus infection</term><term>Rubella virus serology</term><term>Sera serum</term><term>Serum</term><term>Shortridge</term><term>Titrated</term><term>Titre</term><term>Virus</term><term>West nile</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Immune and non-immune rubella sera were examined in the haemagglutination inhibition (HAI) test to evaluate the removal of lipoprotein non-specific inhibitors (NSI) of haemagglutination after kaolin and manganous chloride/heparin treatments. The sera were titrated fresh or after lipoprotein deterioration brought about by ageing the samples at 4 degrees C for various periods of time and by freezing at -20 degrees C with subsequent thawing. Deterioration was seen as altered electrophoretic mobility while the lipoproteins in treated sera were detected by indicator arboviruses whose haemagglutination is known to be strongly inhibited by the native macromolecules. After both treatments, notably manganous chloride/heparin, residual NSI activity was detected in deteriorated samples, particularly with group B arboviruses such as Japanese encephalitis and west Nile viruses but generally less so or not at all with the group A arboviruses employed. Absolutely fresh sera are considered highly desirable for rubella virus HAI assay, and it is suggested that the efficiency of lipoprotein NSI removal regardless of treatment protocol could be monitored in parallel HAI tests using carefully chosen indicator arboviruses. This could be done in conjunction with density gradient centrifugation of doubtful sera should ultracentrifuging facilities be available. The suitability of the monitoring procedure would be dependent to some extent on whether certain arboviruses are known to be endemic in a particular area.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Shortridge, K F" sort="Shortridge, K F" uniqKey="Shortridge K" first="K F" last="Shortridge">K F Shortridge</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A89 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002A89 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:DD8C76345B1ECF4527F15355BE9C5E11C51A5454
|texte= Rubella virus serology:detection of residual lipoprotein inhibitors of haemagglutination using sensitive indicator arboviruses.
}}
| This area was generated with Dilib version V0.6.33. |  |